The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element
Cited 4 times inCited 4 times in
- The promoter activity of the phospholipase C-gamma 2 gene is regulated by a cell-type-specific control element
- Lee, SJ; Bahk, YY; Yun, DH; Lee, HJ; Lee, YH; Ryu, SH; Suh, Pann-Ghill
- MURINE LYMPHOCYTES-B; TYROSINE KINASE; ELEVATED CONTENT; C ISOZYMES; TRANSCRIPTION; EXPRESSION; PROTEIN; DNA; DIFFERENTIATION; PHOSPHORYLATION
- Issue Date
- MARY ANN LIEBERT INC
- DNA AND CELL BIOLOGY, v.16, no.4, pp.485 - 492
- We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma 2 gene. The proximal upstream region is highly CC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts. Multiple transcription initiation sites were identified by primer extension analysis, Based on the transient transfection assays, the major transcriptional activation element was identified between -183 and +43 (G2SE) and a transcriptional repressive element was found between -303 and -184 (G2RE). The expression of PLC-gamma 2 in various cell lines was examined using monoclonal anti-PLC-gamma 2 antibody, PLC-gamma 2 was highly expressed in B-cell lines such as Daudi, SP2, and Ramos cells, whereas it existed at very low levels in Jurkat, 3T3-L1, NBL-7, and C6Bu-1 cells. Moderate levels of PLC-gamma 2 were also detected in C2C12, P19, U937, HL60, A431, and PC12 cells. The 4-kb genomic fragment upstream of -1,654 was able to activate transcription from the PLC-gamma 2 promoter in Daudi and C2C12 cells, but not in Jurkat cells, which is consistent with the PLC-gamma 2 protein expression levels in those cell lines. These results suggest that the cell-type-specific expression of PLC-gamma 2 might be attributed to the transcriptional regulation by the upstream cis-element.
- ; Go to Link
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.