BROWSE

Related Researcher

Author's Photo

Lim, Chunghun
Neurogenetics & Ribonomics Laboratory (The Lim Lab)
Research Interests
  • Ribosome Biology, Translation, Neurodegeneration, Behavioral Genetics

ITEM VIEW & DOWNLOAD

The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo

Cited 71 times inthomson ciCited 73 times inthomson ci
Title
The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo
Author
Kim, SehoKo, JaewonShin, HyewonLee, Jae-RanLim, ChunghunHan, Jin-HeeAltrock, Wilko D.Garner, Craig C.Gundelfinger, Eckart D.Premont, Richard T.Kaang, Bong-KiunKim, Eunjoon
Issue Date
2003-02
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.8, pp.6291 - 6300
Abstract
The cytoskeletal matrix assembled at active zones (CAZ) is implicated in defining neurotransmitter release sites. However, little is known about the molecular mechanisms by which the CAZ is organized. Here we report a novel interaction between Piccolo, a core component of the CAZ, and GIT proteins, multidomain signaling integrators with GTPase-activating protein activity for ADP-ribosylation factor small GTPases. A small region (similar to150 amino acid residues) in Piccolo, which is not conserved in the closely related CAZ protein Bassoon, mediates a direct interaction with the Spa2 homology domain (SHD) domain of GIT1. Piccolo and GIT1 colocalize at synaptic sites in cultured neurons. In brain, Piccolo forms a complex with GIT1 and various GIT-associated proteins, including betaPIX, focal adhesion kinase, liprin-alpha, and paxillin. Point mutations in the SHD of GIT1 differentially interfere with the association of GIT1 with Piccolo, betaPIX, and focal adhesion kinase, suggesting that these proteins bind to the SHD by different mechanisms. Intriguingly, GIT proteins form homo- and heteromultimers through their C-terminal G-protein-coupled receptor kinase-binding domain in a tail-to-tail fashion. This multimerization enables GIT1 to simultaneously interact with multiple SHD-binding proteins including Piccolo and betaPIX. These results suggest that, through their multimerization and interaction with Piccolo, the GIT family proteins are involved in the organization of the CAZ.
URI
https://scholarworks.unist.ac.kr/handle/201301/9841
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037458544
DOI
10.1074/jbc.M212287200
ISSN
0021-9258
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU