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Lim, Mi Hee
MetalloNeuroChemistry Lab (MNCL)
Research Interests
  • Neurodegenerative disease, small molecule design, network between metal, proteins, and ROS

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Abnormal metal levels in the primary visual pathway of the DBA/2J mouse model of glaucoma

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Title
Abnormal metal levels in the primary visual pathway of the DBA/2J mouse model of glaucoma
Author
DeToma, Alaina S.Dengler-Crish, Christine M.Deb, AniruddhaBraymer, Joseph J.Penner-Hahn, James E.Van Der Schyf, Cornelis J.Lim, Mi HeeCrish, Samuel D.
Issue Date
2014-12
Publisher
SPRINGER
Citation
BIOMETALS, v.27, no.6, pp.1291 - 1301
Abstract
The purpose of this study was to determine metal ion levels in central visual system structures of the DBA/2J mouse model of glaucoma. We used inductively coupled plasma mass spectrometry (ICPMS) to measure levels of iron (Fe), copper (Cu), zinc (Zn), magnesium (Mg), manganese (Mn), and calcium (Ca) in the retina and retinal projection of 5-month (pre-glaucomatous) and 10-month (glaucomatous) old DBA/2J mice and age-matched C57BL/6J controls. We used microbeam X-ray fluorescence (μ-XRF) spectrometry to determine the spatial distribution of Fe, Zn, and Cu in the superior colliculus (SC), which is the major retinal target in rodents and one of the earliest sites of pathology in the DBA/2J mouse. Our ICP-MS experiments showed that glaucomatous DBA/2J had lower retinal Fe concentrations than pre-glaucomatous DBA/2J and age-matched C57BL/6J mice. Pre-glaucomatous DBA/2J retina had greater Mg, Ca, and Zn concentrations than glaucomatous DBA/2J and greater Mg and Ca than age-matched controls. Retinal Mn levels were significantly deficient in glaucomatous DBA/2J mice compared to agedmatched C57BL/6J and pre-glaucomatous DBA/2J mice. Regardless of age, the SC of C57BL/6J mice contained greater Fe, Mg, Mn, and Zn concentrations than the SC of DBA/2J mice. Greater Fe concentrations were measured by μ-XRF in both the superficial and deep SC of C57BL/6J mice than in DBA/2J mice. For the first time, we show direct measurement of metal concentrations in central visual system structures affected in glaucoma and present evidence for strain-related differences in metal content that may be specific to glaucomatous pathology.
URI
https://scholarworks.unist.ac.kr/handle/201301/9407
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84911918602
DOI
10.1007/s10534-014-9790-z
ISSN
0966-0844
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PHY_Journal Papers
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