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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Origin-dependent neural cell identities in differentiated human iPSCs in vitro and after transplantation into the mouse brain

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Title
Origin-dependent neural cell identities in differentiated human iPSCs in vitro and after transplantation into the mouse brain
Author
Hargus, GunnarEhrlich, MarcArauzo-Bravo, Marcos J.Hemmer, KathrinHallmann, Anna-LenaReinhardt, PeterKim, Kee-PyoAdachi, KenjiroSantourlidis, SimeonGhanjati, FouedFauser, MareikeOssig, ChristianaStorch, AlexanderKim, Jeong BeomSchwamborn, Jens C.Sterneckert, JaredScholer, Hans R.Kuhlmann, TanjaZaehres, Holm
Issue Date
2014-09
Publisher
Cell Press
Citation
CELL REPORTS, v.8, no.6, pp.1697 - 1703
Abstract
The differentiation capability of induced pluripotent stem cells (iPSCs) toward certain cell types for disease modeling and drug screening assays might be influenced by their somatic cell of origin. Here, we have compared the neural induction of human iPSCs generated from fetal neural stem cells (fNSCs), dermal fibroblasts, or cord blood CD34+ hematopoietic progenitor cells. Neural progenitor cells (NPCs) and neurons could be generated at similar efficiencies from all iPSCs. Transcriptomics analysis of the whole genome and of neural genes revealed a separation of neuroectoderm-derived iPSC-NPCs from mesoderm-derived iPSC-NPCs. Furthermore, we found genes that were similarly expressed in fNSCs and neuroectoderm, but not in mesoderm-derived iPSC-NPCs. Notably, these neural signatures were retained after transplantation into the cortex ofmice and paralleled with increased survival of neuroectoderm-derived cells invivo. These results indicate distinct origin-dependent neural cell identities in differentiated human iPSCs both invitro and invivo.
URI
https://scholarworks.unist.ac.kr/handle/201301/9281
DOI
10.1016/j.celrep.2014.08.014
ISSN
2211-1247
Appears in Collections:
BME_Journal Papers
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