Two-dimensional infrared spectroscopy of isotopomers of an alanine rich alpha-helix
|dc.contributor.author||Kim, Yung Sam||ko|
|dc.identifier.citation||JOURNAL OF PHYSICAL CHEMISTRY B, v.108, no.29, pp.10415 - 10427||ko|
|dc.description.abstract||The two-dimensional infrared spectra of a series of doubly isotopically substituted 25-residue α-helices were measured with femtosecond three pulse infrared time domain interferometry. The insertion of 13C= 16O and 13C= 18O labels at known residues on the helix permitted the vibrational couplings between different amide I' modes separated by one, two, and three residues to be measured. The 2D IR signal of one residue in 25 was readily studied, confirming this approach is applicable to labeled proteins. We identified the couplings between each pair of isotopomer levels and between them and the helix exciton band states: the 2D IR spectra proved that the amide vibrations of the α-helix are delocalized. Cross-peaks, originating from the coupling of the isotopomer pairs, were systematically analyzed. Besides the separated pair modeling and second-order perturbation theory estimates, the experimental results were compared in detail with a full matrix diagonalization simulation based on averaged Hamiltonian matrices that represent the amide I' vibrator's one- and two-exciton states. The main features of the 2D IR spectra could be predicted by this modeling. The experimental results were in good agreement with a set of couplings that were derived from transition chargetransition charge interactions for all but the nearest neighbors, for which the coupling is more influenced by through-bond interactions between the adjacent amide groups. The possible ranges of the magnitudes of the three largest coupling constants β 12, β 13, and β 14 were explored by various approaches to be within a few cm -1 accuracy of a preferred set of absolute values and their associated error bars: |β 12| = 8.5 ± 1.8, |β 13| = 5.4 ± 1.0, and |β 14| = 6.6 ± 0.8 cm -1. The signs were independently indicated to be β 12 > 0, β 13 < 0, and β 14 < 0.||ko|
|dc.publisher||AMER CHEMICAL SOC||ko|
|dc.title||Two-dimensional infrared spectroscopy of isotopomers of an alanine rich alpha-helix||ko|
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