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Kwon, Oh Hoon
Ultrafast Laser Spectroscopy and Nano-microscopy (ULSaN Lab)
Research Interests
  • Femtochemistry/biology, 4D electron microscopy, structural dynamics, materials phenomena

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Molecular tripods showing fluorescence enhancement upon binding to streptavidin

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Title
Molecular tripods showing fluorescence enhancement upon binding to streptavidin
Author
Kim, TWYoon, HYPark, JHKwon, Oh HoonJang, DJHong, JI
Keywords
POLYPYRIDINE BIOTIN COMPLEXES; POLY(ETHYLENE GLYCOL) SPACERS; INTRAMOLECULAR DIMERS; LUMINESCENCE ENHANCEMENT; AVIDIN; PROBES; CYCLODEXTRIN; CONJUGATE; BIOFLUIDS; STRATEGY
Issue Date
2005-01
Publisher
AMER CHEMICAL SOC
Citation
ORGANIC LETTERS, v.7, no.1, pp.111 - 114
Abstract
We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.
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DOI
10.1021/ol047801h
ISSN
1523-7060
Appears in Collections:
PHY_Journal Papers
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