Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin
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- Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin
- Kwon, Tae-Hyuk; Kwon, Jongchul; Hong, Jong-In
- LUMINESCENCE ENHANCEMENT; PHOTOPHYSICAL PROPERTIES; LABELING REAGENTS; BIOTIN COMPLEXES
- Issue Date
- AMER CHEMICAL SOC
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.130, no.12, pp.3726 - 3727
- A neutral tripod system for biotin-avidin assays, which is composed of biotin, FIrpic as an energy acceptor, and mCP as an energy donor, offers remarkable sensitivity over traditional transition metal based protein probes due to the intramolecular energy transfer and increased hydrophobicity associated with the avidin binding site and neutral probe 1.
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