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Lim, Mi Hee
MetalloNeuroChemistry Lab (MNCL)
Research Interests
  • Neurodegenerative disease, small molecule design, network between metal, proteins, and ROS

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DNA strand cleavage near a CC mismatch directed by a metalloinsertor

Cited 11 times inthomson ciCited 3 times inthomson ci
Title
DNA strand cleavage near a CC mismatch directed by a metalloinsertor
Author
Lim, Mi HeeLau, Irvin H.Barton, Jacqueline K.
Keywords
RHODIUM INTERCALATOR; CHEMICAL NUCLEASES; BASE MISMATCHES; REPAIR; RECOGNITION; COMPLEXES; MECHANISMS; SCISSION; BIOLOGY; BINDING
Issue Date
2007-11
Publisher
AMER CHEMICAL SOC
Citation
INORGANIC CHEMISTRY, v.46, no.23, pp.9528 - 9530
Abstract
Reagents for recognition and efficient cleavage of mismatched DNA without photoactivation were designed. They contain a combination of a mismatch-directing metalloinsertor, [Rh(bpy)2(chrysi)]3+ (bpy = 2,2′-bipyridyl, chrysi = 5,6-chrysenequinone diimine), and an oxidative cleavage functionality, [Cu(phen)2]+ (Cu). Both unconjugated (Rh+Cu) and conjugated (Rh-Cu) frameworks of the Rh insertor and Cu were prepared. Compared to Cu, both constructs Rh+Cu and Rh-Cu exhibit efficient site-specific DNA scission only with mismatched DNA, confirmed by experiments with 32P-labeled oligonucleotides. Furthermore, these studies indicate that DNA cleavage occurs near the mismatch in the minor groove and on both strands. Interestingly, the order of reactivity of the three systems with a CC mismatch is Rh+Cu > Rh-Cu ≫ Cu. Rh binding appears to direct Cu reactivity with or without tethering. These results illustrate advantages and disadvantages in bifunctional conjugation.
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DOI
10.1021/ic701598k
ISSN
0020-1669
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PHY_Journal Papers
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