File Download

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

이창수

Lee, Changsoo
Applied Biotechnology Lab for Environment
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

Author(s)
Dueholm, Morten Kam DahlAndersen, Kasper SkytteKorntved, Anne-Kirstine C.Rudkjobing, VibekeAlves, MadalenaBajon-Fernandez, YadiraBatstone, DamienButler, CaitlynCruz, Mercedes CeciliaDavidsson, AsaErijman, LeonardoHolliger, ChristofKoch, KonradKreuzinger, NorbertLee, ChangsooLyberatos, GerasimosMutnuri, SrikanthO'Flaherty, VincentOleskowicz-Popiel, PiotrPokorna, DanaRajal, VeronicaRecktenwald, MichaelRodriguez, JorgeSaikaly, Pascal E.Tooker, NickVierheilig, JuliaDe Vrieze, JoWurzbacher, ChristianNielsen, Per Halkjaer
Issued Date
2024-06
DOI
10.1038/s41467-024-49641-y
URI
https://scholarworks.unist.ac.kr/handle/201301/83317
Citation
NATURE COMMUNICATIONS, v.15, no.1, pp.5361
Abstract
Anaerobic digestion of organic waste into methane and carbon dioxide (biogas) is carried out by complex microbial communities. Here, we use full-length 16S rRNA gene sequencing of 285 full-scale anaerobic digesters (ADs) to expand our knowledge about diversity and function of the bacteria and archaea in ADs worldwide. The sequences are processed into full-length 16S rRNA amplicon sequence variants (FL-ASVs) and are used to expand the MiDAS 4 database for bacteria and archaea in wastewater treatment systems, creating MiDAS 5. The expansion of the MiDAS database increases the coverage for bacteria and archaea in ADs worldwide, leading to improved genus- and species-level classification. Using MiDAS 5, we carry out an amplicon-based, global-scale microbial community profiling of the sampled ADs using three common sets of primers targeting different regions of the 16S rRNA gene in bacteria and/or archaea. We reveal how environmental conditions and biogeography shape the AD microbiota. We also identify core and conditionally rare or abundant taxa, encompassing 692 genera and 1013 species. These represent 84-99% and 18-61% of the accumulated read abundance, respectively, across samples depending on the amplicon primers used. Finally, we examine the global diversity of functional groups with known importance for the anaerobic digestion process. Anaerobic digesters play an important role in biodegradation. In the MiDAS 5 project, the authors use global 16S rRNA sequencing to expand the microbial reference database, improving taxonomic classification and revealing how environmental factors and geography shape microbial communities in anaerobic digesters.
Publisher
NATURE PORTFOLIO
ISSN
2041-1723
Keyword
SYNTROPHUS-ACIDITROPHICUSPROPIONATE OXIDATIONSMITHELLA-PROPIONICAMICROBIAL COMMUNITYGEN. NOV.SP. NOV.SP-NOV.FATTY-ACIDSGENOMEASSOCIATION

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.