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박성훈

Park, Sunghoon
Biochemical Engineering Lab.
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Efficient bioproduction of poly(3-hydroxypropionate) homopolymer using engineered Escherichia coli strains

Author(s)
Gaur, Vivek KumarNguyen-Vo, Thuan PhuIslam, TayyabBassey, Bassey FridayKim, MiriAinala, Satish KumarShin, KyusoonPark, Sunghoon
Issued Date
2024-04
DOI
10.1016/j.biortech.2024.130469
URI
https://scholarworks.unist.ac.kr/handle/201301/82702
Citation
BIORESOURCE TECHNOLOGY, v.397, pp.130469
Abstract
This study focuses on the development of a scalable method for producing poly(3-hydroxypropionate), a homopolymer with significant physico-mechanical properties, through the use of metabolically-engineered Escherichia coli K12 (MG1655) and externally supplied 3-hydroxypropionate. The polymer synthesis pathway was established and optimized through synthetic biology techniques, including the effects of overexpressing phasin and cell division proteins. The optimized strain achieved unprecedented production titers of 9.5 g/L in flask cultures and 80 g/L in fed-batch bioreactors within 45 h. The analysis of poly(3-hydroxypropionate) polymer properties revealed it possesses excellent elasticity (Young's modulus < 6 MPa) and tensile strength (similar to 80 MPa), positioning it within the category of elastomers or flexible plastics. These findings suggest a viable path for the sustainable, large-scale production of the poly(3-hydroxypropionate) biopolymer.
Publisher
ELSEVIER SCI LTD
ISSN
0960-8524
Keyword (Author)
Metabolic engineeringP(3-HP) productionBioplasticsPhasinPhysico-mechanical property
Keyword
MOLECULAR-WEIGHTPOLY(3-HYDROXYBUTYRATE)GLYCEROLCULTUREPOLYHYDROXYALKANOATEACCUMULATIONCOPOLYMERSYNTHASEPHB

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