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Choi, Sung-Deuk
Environmental Analytical Chemistry Lab (EACL)
Research Interests
  • Persistent organic pollutants, Environmental Analysis and monitoring, Multimedia modeling, Fine particle

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Isolation and characterization of a cell-associated protein of Bacillus pumilus PH-01

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Title
Isolation and characterization of a cell-associated protein of Bacillus pumilus PH-01
Author
Hong, HBChang, YSChoi, Sung-DeukNam, IHLee, YE
Issue Date
2001-08
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.56, no.3-4, pp.402 - 405
Abstract
A cell-associated protein released from Bacillus pumilus PH-01 showed an affinity for some dioxins, like 1,2,3,4-tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,4-tetrachlorodibenzofuran (TCDF), and the concentration of the protein increased when B. pumilus PH-01 was boiled in minimal salts medium. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-mass spectrometry revealed that the boiled culture supernatant contained a major protein with a molecular mass of 5,313.4 Da. The adsorption behavior of the protein for 1,2,3,4-TCDD and 1,2,3,4-TCDF was examined by digesting it with proteinase K and trypsin, showing that the proteolyzed protein lost the ability to adsorb the compounds. The amino acid sequence of the protein was determined by automated Edman degradation and tandem mass spectrometry. A search of the protein databases showed no existence of proteins with an homologous sequence.
URI
https://scholarworks.unist.ac.kr/handle/201301/8217
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034887056
DOI
10.1007/s002530100638
ISSN
0175-7598
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