Full metadata record
DC Field | Value | Language |
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dc.citation.number | 1 | - |
dc.citation.startPage | 142 | - |
dc.citation.title | CELL COMMUNICATION AND SIGNALING | - |
dc.citation.volume | 22 | - |
dc.contributor.author | Manandhar, Laxman | - |
dc.contributor.author | Dutta, Raghbendra Kumar | - |
dc.contributor.author | Devkota, Pradeep | - |
dc.contributor.author | Chhetri, Arun | - |
dc.contributor.author | Wei, Xiaofan | - |
dc.contributor.author | Park, Channy | - |
dc.contributor.author | Kwon, Hyug Moo | - |
dc.contributor.author | Park, Raekil | - |
dc.date.accessioned | 2024-04-04T10:05:11Z | - |
dc.date.available | 2024-04-04T10:05:11Z | - |
dc.date.created | 2024-04-03 | - |
dc.date.issued | 2024-02 | - |
dc.description.abstract | BackgroundCalcium is a ubiquitous intracellular messenger that regulates the expression of various genes involved in cell proliferation, differentiation, and motility. The involvement of calcium in diverse metabolic pathways has been suggested. However, the effect of calcium in peroxisomes, which are involved in fatty acid oxidation and scavenges the result reactive oxygen species (ROS), remains elusive. In addition, impaired peroxisomal ROS inhibit the mammalian target of rapamycin complex 1 (mTORC1) and promote autophagy. Under stress, autophagy serves as a protective mechanism to avoid cell death. In response to oxidative stress, lysosomal calcium mediates transcription factor EB (TFEB) activation. However, the impact of calcium on peroxisome function and the mechanisms governing cellular homeostasis to prevent diseases caused by calcium deficiency are currently unknown.MethodsTo investigate the significance of calcium in peroxisomes and their roles in preserving cellular homeostasis, we established an in-vitro scenario of calcium depletion.ResultsThis study demonstrated that calcium deficiency reduces catalase activity, resulting in increased ROS accumulation in peroxisomes. This, in turn, inhibits mTORC1 and induces pexophagy through TFEB activation. However, treatment with the antioxidant N-acetyl-l-cysteine (NAC) and the autophagy inhibitor chloroquine impeded the nuclear translocation of TFEB and attenuated peroxisome degradation.ConclusionsCollectively, our study revealed that ROS-mediated TFEB activation triggers pexophagy during calcium deficiency, primarily because of attenuated catalase activity. We posit that calcium plays a significant role in the proper functioning of peroxisomes, critical for fatty-acid oxidation and ROS scavenging in maintaining cellular homeostasis. These findings have important implications for signaling mechanisms in various pathologies, including Zellweger's syndrome and ageing. | - |
dc.identifier.bibliographicCitation | CELL COMMUNICATION AND SIGNALING, v.22, no.1, pp.142 | - |
dc.identifier.doi | 10.1186/s12964-024-01524-x | - |
dc.identifier.issn | 1478-811X | - |
dc.identifier.scopusid | 2-s2.0-85185619791 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/81957 | - |
dc.identifier.wosid | 001169401600003 | - |
dc.language | 영어 | - |
dc.publisher | BMC | - |
dc.title | TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Calcium | - |
dc.subject.keywordAuthor | Peroxisome | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Catalase | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | TFEB | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | MUSCLE | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | PEROXISOMES | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | MTOR | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | PATHWAY | - |
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