Construction and Enhancement of E. coli Biosensor for Detection of Landmine

Abstract

As is well known, globally buried landmines cause serious problems such as human casualties and environmental pollution. An accurate and safe method of detecting landmines is needed, but no method has been developed to effectively detect various kinds of mines. Based on previous studies, a biosensor to detect DNT has been constructed by expressing gfp +, an enhanced fluorescence protein gene under the Pyqjfmut, a strong DNT-responsive promoter. However, the biosensor showed a weak signal at 3 ppb of low DNT concentration with high leaky expression. To solve these problems, genes related to DNT metabolism were identified through Tn-library screening and ASKA library screening to engineer them in order to improve the overall performance of the biosensor.