Bio-inks should not only safely deliver viable cells into a designated area, but also provide proper environments for enhanced regeneration of artificial tissue and wound healing. Various kinds of methods have been introduced to enhance biocompatibility of bio-ink by developing a biomimetic material. Among them, decellularized ECM (dECM) method is one of the most attractive ways because it can produce a material composed of ECM components of native tissue. dECM based hydrogels have been introduced as bio-ink. However, although the bio-ink had good biocompatibility, the researches failed in demonstrating 3D printability of the bio-ink. Here, we introduce a novel strategy to enhance a printability of dECM based bio-ink. A dECM powder came from pig liver was prepared. Then it was mixed with gelatin solution to prepare a bio-ink. The material was applied into biocompatibility and printability test to demonstrate its usefulness. As a result, cyto-compatibility of new bio-ink was similar with dECM hydrogel. On the other hand, enhanced printability of the new material was shown in the 2D and 3D patterning test. These results successfully demonstrate that the new strategy can apply in developing various types of dECM based bio-ink that supports good biocompatibility and printability.