3D 바이오프린팅된 환자맞춤형 치아구조체 내 치수줄기세포의 선택적 분화 유도

Abstract

Recently, despite of some efforts to develop bioengineered teeth, conventional methods are limited in recapitulating complex external outlines and heterogeneous tissue distribution in teeth. On the other hand, 3D bio-printing is considered to be the most relevant technology for tooth recapitulation in that it can pattern various cells and materials discretely into 3D complex structure. In this paper, we developed a patient specific bioengineered tooth by 3D bio-printing of Dental Pulp Stem Cells (DPSC). First, fibrin based mineralization-tunable bio-ink was developed to mimic composite tissues in teeth, specially mineralized dentin and un-mineralized pulp. It shows excellent cell viability over 90%, and odontogenic differentiation of DPSC varied according to concentration of fibrinogen. Based on the 3D image of a real tooth, we fabricated tooth-like biphasic cellular construct by printing developed heterogeneous bio-inks discretely. In differentiation medium on day 12, it had separated mineralized and un-mineralized tissues mimicking dentin and pulp. The results of this study showed the possibility to fabricate patient-specific bioengineered tooth and its application for dental regeneration. Furthermore, it is expected that the developed printing processes and tunable bio-ink for spatial regulation of stem cell fate will be widely used in tissue engineering and regenerative medicines.