Store-Operated Ca2+ Entry is Essential for Brown Adipogenesis.

Abstract

Brown adipocytes are important for non-shivering thermogenesis, lipid homeostasis, and anti-obesity. Ca2+ signaling is essential for adipocytes differentiation, adipogenesis. However, underlying mechanism and detailed function of Ca2+ channel in brown adipogenesis remain elusive. Here, we showed that Store-operated Ca2+ entry (SOCE), the major Ca2+ entry pathway in non-excitable cells, is essential for brown adipocytes differentiation. We established brown pre-adipocytes (B8 cells) from mouse interscapular brown fat pads. We demonstrated that the mRNA expression level of SOCE modulators, Orai and STIM, changed during brown adipocytes differentiation. In addition, we investigated the function of SOCE in B8 cells with Ca2+ imaging, and CRISPR mediated knockout approach. we figured out that deletion of SOCE modulators promoted the brown fat differentiation by altering Ca2+ homeostasis in B8 cells and mouse. In conclusion, this study suggests that SOCE is an unsung regulator of Ca2+ signaling during brown fat differentiation.