Mix and go Delivery Platform Based on Protein Coated Nanoparticle for Targeted delivery

Abstract

Antibody-mediated target delivery systems have received great attention because targeting antibodies have extremely high binding affinity and specificity for their target molecules as well as a variety of antibodies against virtually any desired targets can be readily generated on demand. However, conjugation of the antibodies to nanoparticle based delivery systems generally require complicated chemical modification steps which often result in impairment of antibody’s targeting capability. We, herein, reported Mix-and-Go delivery systems based on the bioconjugation between the fusion protein coated silica nanoparticle and antibody. We prepared an adaptor fusion protein, GST-ABD, which was constructed by genetically fusing glutathione-S-transferase (GST) and antibody-binding domain (ABD) of Protein A and used it to mediate efficient complexation between targeting antibodies and silica nanoparticle without any complicated modifications. GST-ABDs were successfully anchored with specific alignment on the surface of nanoparticle through the GST active site target linker (GSH) and the GST-ABD-NP efficiently captured various targeting antibodies in an orientation-controlled manner through the selective interaction between displayed ABDs and the Fc region of the antibodies. Their target-specific delivery ability by surface-displayed targeting antibodies was confirmed by fluorescence cell imaging and cell viability tests. This approach may offer the opportunity to develop new types of universal drug delivery platform which can be plugged with any targeting antibodies on demand without any complicated chemical modification and used for desired targets on demand.