Multivalent Interaction using disulfide formation-induced Self-Assembly inside Mitochondria for Senolytic Effect

Abstract

The intracellular biomacromolecules including proteins, DNA, and polysaccharides, play an important role to perform cellular function. Theses macromolecules are synthesized by multicomponent self-assembly of building blocks such as amino acids, nucleotides, and monossacharides. Such an assembly relies on interaction of individual building blocks to create complex and high ordered structure. By mimicking the natural system, we found that formation of disulfide bond give a great platform to form multicomponent assembled structure for multivalent interaction with bio-macromolecules. Thus, we developed multicomponent self-assembly using disulfide bond to interact with mitochondrial membrane via multivalent interaction for inducing dysfunction of mitochondria, leading to apoptosis of senescent cells specifically. This polymerization shows great potential for senolytic treatment with selective removal of senescent cells.