Fast, three-dimensional super-resolution imaging of live cells
Cited 241 times inCited 228 times in
- Fast, three-dimensional super-resolution imaging of live cells
- Jones, Sara A.; Shim, Sang-Hee; He, Jiang; Zhuang, Xiaowei
- PHOTOACTIVATED LOCALIZATION MICROSCOPY; OPTICAL RECONSTRUCTION MICROSCOPY; LIVING CELLS; FLUORESCENCE MICROSCOPY; STRUCTURED-ILLUMINATION; DIFFRACTION-LIMIT; FUSION PROTEINS; RESOLUTION; NANOSCOPY; PROBES
- Issue Date
- NATURE PUBLISHING GROUP
- NATURE METHODS, v.8, no.6, pp.499 - U96
- We report super-resolution fluorescence imaging of live cells with high spatiotemporal resolution using stochastic optical reconstruction microscopy (STORM). By labeling proteins either directly or via SNAP tags with photoswitchable dyes, we obtained two-dimensional (2D) and 3D super-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model systems. Bright, fast-switching probes enabled us to achieve 2D imaging at spatial resolutions of 1/425 nm and temporal resolutions as fast as 0.5 s. We also demonstrated live-cell 3D super-resolution imaging. We obtained 3D spatial resolution of 1/430 nm in the lateral direction and 1/450 nm in the axial direction at time resolutions as fast as 1-2 s with several independent snapshots. Using photoswitchable dyes with distinct emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells. These imaging capabilities open a new window for characterizing cellular structures in living cells at the ultrastructural level.
- ; Go to Link
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.