BROWSE

Related Researcher

Author's Photo

Shim, Sang-Hee
Shim Research Lab
Research Interests
  • Super-resolution Optical Imaging
  • Single-molecule Biophysics
  • Ultrastructural Dynamics
  • Single-cell Systems Biology

ITEM VIEW & DOWNLOAD

Fast, three-dimensional super-resolution imaging of live cells

Cited 241 times inthomson ciCited 228 times inthomson ci
Title
Fast, three-dimensional super-resolution imaging of live cells
Author
Jones, Sara A.Shim, Sang-HeeHe, JiangZhuang, Xiaowei
Keywords
PHOTOACTIVATED LOCALIZATION MICROSCOPY; OPTICAL RECONSTRUCTION MICROSCOPY; LIVING CELLS; FLUORESCENCE MICROSCOPY; STRUCTURED-ILLUMINATION; DIFFRACTION-LIMIT; FUSION PROTEINS; RESOLUTION; NANOSCOPY; PROBES
Issue Date
2011-06
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE METHODS, v.8, no.6, pp.499 - U96
Abstract
We report super-resolution fluorescence imaging of live cells with high spatiotemporal resolution using stochastic optical reconstruction microscopy (STORM). By labeling proteins either directly or via SNAP tags with photoswitchable dyes, we obtained two-dimensional (2D) and 3D super-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model systems. Bright, fast-switching probes enabled us to achieve 2D imaging at spatial resolutions of 1/425 nm and temporal resolutions as fast as 0.5 s. We also demonstrated live-cell 3D super-resolution imaging. We obtained 3D spatial resolution of 1/430 nm in the lateral direction and 1/450 nm in the axial direction at time resolutions as fast as 1-2 s with several independent snapshots. Using photoswitchable dyes with distinct emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells. These imaging capabilities open a new window for characterizing cellular structures in living cells at the ultrastructural level.
URI
Go to Link
DOI
10.1038/NMETH.1605
ISSN
1548-7091
Appears in Collections:
BME_Journal Papers
Files in This Item:
2-s2.0-79957823841.pdf Download

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU