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DC Field | Value | Language |
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dc.citation.endPage | 3402 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 3392 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 28 | - |
dc.contributor.author | Kim, M | - |
dc.contributor.author | Lee, W | - |
dc.contributor.author | Park, Jiyoung | - |
dc.contributor.author | Kim, JB | - |
dc.contributor.author | Jang, YK | - |
dc.contributor.author | Seong, RH | - |
dc.contributor.author | Choe, SY | - |
dc.contributor.author | Park, SD | - |
dc.date.accessioned | 2023-12-22T12:06:57Z | - |
dc.date.available | 2023-12-22T12:06:57Z | - |
dc.date.created | 2014-10-13 | - |
dc.date.issued | 2000-09 | - |
dc.description.abstract | Exposure of Schizosaccharomyces pombe cells to UV light results in increased uvi15+ gene expression at both the mRNA and protein levels, leading to elevated cell survival. This UV-induced expression of the uvi15+ gene was reduced in Δsty1 and Δwis1 cells lacking the stress-activated protein kinase pathway, but not in DNA damage checkpoint mutants. To further understand the cellular mechanisms responsible for this UV-induced expression, the transcription rate and mRNA half-life were investigated. Transcription run-on assays revealed that the rate of uvi15+ transcription was increased 1.8-fold regardless of Sty1 when cells were UV irradiated. The half-life of uvi15+ mRNA was also increased 1.5-fold after UV irradiation, but it was decreased in the Δsty1 background for both basal and UV-induced mRNAs, indicating that the stress-activated MAPK cascade can mediate UV-induced gene expression by increasing mRNA half-life. Deletion analyses identified a 54 nt element downstream of the distal poly(A) site, which was involved in the increased half-life of uvi15+ mRNA. These results suggest that both Sty1 and the 3'-regulatory element regulate UV-induced expression of the uvi15+ gene at the post-transcriptional level. | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.28, no.17, pp.3392 - 3402 | - |
dc.identifier.doi | 10.1093/nar/28.17.3392 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-0034284362 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7157 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034284362 | - |
dc.identifier.wosid | 000089078900026 | - |
dc.language | 영어 | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | The stress-activated MAP kinase Sty1/Spc1 and a 3 '-regulatory element mediate UV-induced expression of the uvi15+ gene at the post-transcriptional level | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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