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DC Field | Value | Language |
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dc.citation.endPage | 10183 | - |
dc.citation.number | 25 | - |
dc.citation.startPage | 10178 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 108 | - |
dc.contributor.author | Lamboy, Jorge A. | - |
dc.contributor.author | Kim, Hajin | - |
dc.contributor.author | Lee, Kyung Suk | - |
dc.contributor.author | Ha, Taekjip | - |
dc.contributor.author | Komives, Elizabeth A. | - |
dc.date.accessioned | 2023-12-22T06:09:11Z | - |
dc.date.available | 2023-12-22T06:09:11Z | - |
dc.date.created | 2014-10-10 | - |
dc.date.issued | 2011-06 | - |
dc.description.abstract | IκBα is a crucial regulator of NFκB transcription. NFκB-mediated gene activation is robust because levels of free IκBα are kept extremely low by rapid, ubiquitin-independent degradation of newly synthesized IκBα. IκBα has a weakly folded ankyrin repeat 5-6 (AR5-6) region that is critical in establishing its short intracellular half-life. The AR5-6 region of IκBα folds upon binding to NFκB. The NFκB-bound IκBα has a long half-life and requires ubiquitin-targeted degradation. We present single molecule FRET evidence that the native state of IκBα transiently populates an intrinsically disordered state characterized by a more extended structure and fluctuations on the millisecond time scale. Binding to NFκB or introduction of stabilizing mutations in AR 6 suppressed the fluctuations, whereas higher temperature or small amounts of urea increased them. The results reveal that intrinsically disordered protein regions transition between collapsed and extended conformations under native conditions. | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.25, pp.10178 - 10183 | - |
dc.identifier.doi | 10.1073/pnas.1102226108 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.scopusid | 2-s2.0-79959946164 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7107 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79959946164 | - |
dc.identifier.wosid | 000291857500035 | - |
dc.language | 영어 | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.title | Visualization of the nanospring dynamics of the I kappa B alpha ankyrin repeat domain in real time | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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