There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | + | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 623 | - |
dc.citation.title | NATURE STRUCTURAL & MOLECULAR BIOLOGY | - |
dc.citation.volume | 19 | - |
dc.contributor.author | Cisse, Ibrahim I. | - |
dc.contributor.author | Kim, Hajin | - |
dc.contributor.author | Ha, Taekjip | - |
dc.date.accessioned | 2023-12-22T05:07:57Z | - |
dc.date.available | 2023-12-22T05:07:57Z | - |
dc.date.created | 2014-10-10 | - |
dc.date.issued | 2012-06 | - |
dc.description.abstract | Sequence recognition through base-pairing is essential for DNA repair and gene regulation, but the basic rules governing this process remain elusive. In particular, the kinetics of annealing between two imperfectly matched strands is not well characterized, despite its potential importance in nucleic acid-based biotechnologies and gene silencing. Here we use single-molecule fluorescence to visualize the multiple annealing and melting reactions of two untethered strands inside a porous vesicle, allowing us to precisely quantify the annealing and melting rates. The data as a function of mismatch position suggest that seven contiguous base pairs are needed for rapid annealing of DNA and RNA. This phenomenological rule of seven may underlie the requirement for seven nucleotides of complementarity to seed gene silencing by small noncoding RNA and may help guide performance improvement in DNA- and RNA-based bio- and nanotechnologies, in which off-target effects can be detrimental. | - |
dc.identifier.bibliographicCitation | NATURE STRUCTURAL & MOLECULAR BIOLOGY, v.19, no.6, pp.623 - + | - |
dc.identifier.doi | 10.1038/nsmb.2294 | - |
dc.identifier.issn | 1545-9993 | - |
dc.identifier.scopusid | 2-s2.0-84861892611 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7105 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84861892611 | - |
dc.identifier.wosid | 000304958200010 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | A rule of seven in Watson-Crick base-pairing of mismatched sequences | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | NEAREST-NEIGHBOR THERMODYNAMICS | - |
dc.subject.keywordPlus | NUCLEIC-ACID HYBRIDIZATION | - |
dc.subject.keywordPlus | TARGET RECOGNITION | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDE | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.