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DC Field | Value | Language |
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dc.citation.endPage | 7418 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 7414 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 108 | - |
dc.contributor.author | Hwang, Helen | - |
dc.contributor.author | Kim, Hajin | - |
dc.contributor.author | Myong, Sua | - |
dc.date.accessioned | 2023-12-22T06:10:59Z | - |
dc.date.available | 2023-12-22T06:10:59Z | - |
dc.date.created | 2014-10-10 | - |
dc.date.issued | 2011-05 | - |
dc.description.abstract | Single-molecule FRET has been widely used for monitoring protein-nucleic acids interactions. Direct visualization of the interactions, however, often requires a site-specific labeling of the protein, which can be circuitous and inefficient. In addition, FRET is insensitive to distance changes in the 0-3-nm range. Here, we report a systematic calibration of a single molecule fluorescence assay termed protein induced fluorescence enhancement. This method circumvents protein labeling and displays a marked distance dependence below the 4-nm distance range. The enhancement of fluorescence is based on the photophysical phenomenon whereby the intensity of a fluorophore increases upon proximal binding of a protein. Our data reveals that the method can resolve as small as a single base pair distance at the extreme vicinity of the fluorophore, where the enhancement is maximized. We demonstrate the general applicability and distance sensitivity using (a) a finely spaced DNA ladder carrying a restriction site for BamHI, (b) RNA translocation by DExH enzyme RIG?I, and (c) filament dynamics of RecA on single-stranded DNA. The high spatio-temporal resolution data and sensitivity to short distances combined with the ability to bypass protein labeling makes this assay an effective alternative or a complement to FRET. | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.108, no.18, pp.7414 - 7418 | - |
dc.identifier.doi | 10.1073/pnas.1017672108 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.scopusid | 2-s2.0-79956335271 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7083 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79956335271 | - |
dc.identifier.wosid | 000290203100032 | - |
dc.language | 영어 | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.title | Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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