Bryostatin-1 in combination with calcium ionophore promotes the maturation of human umbilical cord-blood monocyte-derived dendritic cells capable of activating neonatal alloreactive T cells
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- Bryostatin-1 in combination with calcium ionophore promotes the maturation of human umbilical cord-blood monocyte-derived dendritic cells capable of activating neonatal alloreactive T cells
- Do, Yoonkyung; Mainali, E; Nagarkatti, PS; Nagarkatti, M
- Alloreactive T cells; Byrostatin-1; Calcium ionophore; Class II major histocompatability complex; Co-stimulatory molecule; Cord blood; Dendritic cells; Interferon-γ; Maturation; Tumor necrosis factor-α
- Issue Date
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- CELLULAR IMMUNOLOGY, v.231, no.1-2, pp.8 - 13
- We have investigated the effect of bryostatin-1 (Bryo-1) and calcium ionophore (CI) on maturation and functions of DCs generated from adherent cells of cord blood cultured with GM-CSF plus IL-4 (CB-DCs). The CB-DCs treated with Bryo-1 + CI exhibited morphologic characteristics of mature DCs, expressed increased levels of CD1a, CD80, CD83, CD86, and MHC class II as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN-γ production. Treatment of CB-DCs with TNF-α or PMA + CI was less effective. Thus, Bryo-1 + CI promotes maturation of CB-DCs and therefore could be used to enhance the neonatal immune response.
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