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Bryostatin-1 in combination with calcium ionophore promotes the maturation of human umbilical cord-blood monocyte-derived dendritic cells capable of activating neonatal alloreactive T cells
- Author(s)
- Do, Yoonkyung, Mainali, E, Nagarkatti, PS, Nagarkatti, M
- Issued Date
- 2004-09
- Citation
- CELLULAR IMMUNOLOGY, v.231, no.1-2, pp.8 - 13
- Abstract
- We have investigated the effect of bryostatin-1 (Bryo-1) and calcium ionophore (CI) on maturation and functions of DCs generated from adherent cells of cord blood cultured with GM-CSF plus IL-4 (CB-DCs). The CB-DCs treated with Bryo-1 + CI exhibited morphologic characteristics of mature DCs, expressed increased levels of CD1a, CD80, CD83, CD86, and MHC class II as well as enhanced ability to induce proliferation of alloreactive T cells isolated from cord blood and IFN-γ production. Treatment of CB-DCs with TNF-α or PMA + CI was less effective. Thus, Bryo-1 + CI promotes maturation of CB-DCs and therefore could be used to enhance the neonatal immune response.
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- ISSN
- 0008-8749
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