Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins
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- Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins
- Lee, In Su; Lee, Nohyun; Park, Jongnam; Kim, Byung Hyo; Yi, Yong-Weon; Kim, Taeuk; Kim, Tae Kook; Lee, In Hwan; Paik, Seung R.; Hyeon, Taeghwan
- Issue Date
- AMER CHEMICAL SOC
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.128, no.33, pp.10658 - 10659
- Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
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