dc.citation.conferencePlace |
KO |
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dc.citation.conferencePlace |
제주 ICC |
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dc.citation.title |
2023 한국분자세포생물학회 정기학술대회, ICKSMCB |
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dc.contributor.author |
Lee, Youn Young |
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dc.contributor.author |
Kim, Ji Eun |
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dc.contributor.author |
Kim, Min Ji |
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dc.contributor.author |
Lee, Jong Hee |
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dc.contributor.author |
Kim, Sun Uk |
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dc.contributor.author |
Park, Chan Young |
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dc.date.accessioned |
2024-01-09T18:05:09Z |
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dc.date.available |
2024-01-09T18:05:09Z |
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dc.date.created |
2024-01-09 |
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dc.date.issued |
2023-11-06 |
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dc.description.abstract |
Terminal erythropoiesis occurs through a complicated differentiation process that relies on erythropoietin (EPO) signal transduction and intracellular gene regulation. By studying the Orai1 Ca2+channel, we gain mechanistic insights into how EPO-mediated Ca2+signals modulate erythroid gene transcription following the maturation of RBCs. Here, we show that expression of Orai1 decreased in terminally differentiating HUDEP-2 and cord blood (CB)-derived CD71 primary erythroblasts, affecting the Ca2+homeostasis of erythroid cells. Repression of the Orai1 Ca2+channel using mutants promoted RBC differentiation efficiency in HUDEP-2 and iPSCs. In addition, Orai1 inactivation positively manipulated erythroid-specific genes by promoting Krupple-like factor 1 (KLF1) gene expression. We also found that EPO activates the Orai1 channel. Elimination of the EPO stimuli highly improved maturation efficiency, which might cause a decrease in Orai1 activity. Overall, this study demonstrated an unknown function of the Orai1 Ca2+channel in terminal erythropoiesis and improved the understanding of how EPO-mediated Ca2+signaling regulates erythroid gene transcription during RBC differentiation. |
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dc.identifier.bibliographicCitation |
2023 한국분자세포생물학회 정기학술대회, ICKSMCB |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/67928 |
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dc.publisher |
한국분자세포생물학회 |
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dc.title |
Decrease of Orai1 Ca2+ channel primes KLF1 transcription for terminal erythropoiesis |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2023-11-06 |
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