dc.citation.conferencePlace |
KO |
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dc.citation.conferencePlace |
부산 벡스코 |
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dc.citation.title |
한국줄기세포학회 2023 연례학술대회 2023 Annual Meeting of Korean Society for Stem Cell Research |
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dc.contributor.author |
Lee, Youn Young |
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dc.contributor.author |
Kim, Ki Eun |
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dc.contributor.author |
Lee, Hong Hee |
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dc.contributor.author |
Park, Chan Young |
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dc.date.accessioned |
2024-01-09T17:35:08Z |
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dc.date.available |
2024-01-09T17:35:08Z |
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dc.date.created |
2024-01-09 |
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dc.date.issued |
2023-03-16 |
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dc.description.abstract |
Terminal erythropoiesis occurs through a complicated differentiation process that relies on erythropoietin (EPO) signal transduction and intracellular gene regulation. Although recent studies have shown that Ca2+-mediated intracellular signaling may contribute to the proper maturation of erythroid cells, the underlying regulation of the Ca2+ channel remains poorly understood. By studying the Erythroid Ca2+ channel 1 (ECC1), we gained mechanistic insights into how EPO-mediated Ca2+ signals modulate erythroid gene transcription following the maturation of RBCs. Here, we show that expression of ECC1 decreased in terminally differentiating HUDEP-2 and cord blood (CB)-derived CD71 primary erythroblasts, affecting the Ca2+ homeostasis of erythroid cells. Repression of the ECC1 Ca2+ channel using mutants and knock-out of ECC1 promoted RBC differentiation efficiency in HUDEP-2 and iPSCs. In addition, ECC1 inactivation positively manipulated erythroid-specific genes by promoting Krüppel-like factor 1 (KLF1) gene expression. We also found that EPO activates the ECC1 Ca2+ channel, especially in pro- and basophilic-erythroblasts stages. Elimination of the EPO stimuli highly improved maturation efficiency, which might cause a decrease in ECC1 channel activity..Overall, this study demonstrated an unknown function of the ECC1 Ca2+ channel in terminal erythropoiesis and improved the understanding of how EPO-mediated Ca2+ signaling regulates erythroid gene transcription during red blood cell differentiation. |
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dc.identifier.bibliographicCitation |
한국줄기세포학회 2023 연례학술대회 2023 Annual Meeting of Korean Society for Stem Cell Research |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/67926 |
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dc.publisher |
한국줄기세포학회 |
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dc.title |
Decrease of ECC1 Ca2+ channel primes KLF1 transcription for terminal erythropoiesis |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2023-03-16 |
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