BROWSE

Related Researcher

Author's Photo

Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

ITEM VIEW & DOWNLOAD

Catabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: Evidence for involvement of the cyclic AMP receptor protein

Cited 24 times inthomson ciCited 25 times inthomson ci
Title
Catabolite repression of the propionate catabolic genes in Escherichia coli and Salmonella enterica: Evidence for involvement of the cyclic AMP receptor protein
Author
Lee, Sung KukNewman, J.D.Keasling, J.D.
Keywords
TYPHIMURIUM LT2; TRANSCRIPTION ACTIVATION; CARBON METABOLISM; ENCODING ENZYMES; DOWN-REGULATION; RNA-POLYMERASE; PRPBCDE OPERON; PRP LOCUS; PROMOTER; EXPRESSION
Issue Date
2005-04
Publisher
AMER SOC MICROBIOLOGY
Citation
JOURNAL OF BACTERIOLOGY, v.187, no.8, pp.2793 - 2800
Abstract
Previous studies with Salmonella enterica serovar Typhimurium LT2 demonstrated that transcriptional activation of the prpBCDE operon requires the function of transcription factor PrpR, sigma-54, and IHF. In this study, we found that transcription from the prpBCDE and prpR promoters was down-regulated by the addition of glucose or glycerol. indicating that these genes may be regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Targeted mutagenesis of a putative CRP-binding site in the promoter region between prpR and prpBCDE suggested that these genes are under the control of CRP. Furthermore, cells with defects in cya or crp exhibited reduced transcriptional activation of prpR and prpBCDE in Escherichia coli. These results demonstrate that propionate metabolism is subject to catabolite repression by the global transcriptional regulator CRP and that this regulation is effected through control of both the regulator gene prpR and the prpBCDE operon itself. The unique properties of the regulation of these two divergent promoters may have important implications for mechanisms of CRP-dependent catabolite repression acting in conjunction with a member of the sigma-54 family of transcriptional activators.
URI
Go to Link
DOI
10.1128/JB.187.8.2793-2800.2005
ISSN
0021-9193
Appears in Collections:
ECHE_Journal Papers
Files in This Item:
2-s2.0-16844384950.pdf Download

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU