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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

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A propionate-inducible expression system for enteric bacteria

Cited 38 times inthomson ciCited 40 times inthomson ci
Title
A propionate-inducible expression system for enteric bacteria
Author
Lee, Sung KukKeasling, JD
Keywords
SALMONELLA-TYPHIMURIUM LT2; 2-METHYLCITRATE DEHYDRATASE PRPD; ESCHERICHIA-COLI K-12; P-BAD PROMOTER; GENE-EXPRESSION; REGULATED EXPRESSION; PROTEIN EXPRESSION; ARABAD PROMOTER; OPERON; INDUCTION
Issue Date
2005-11
Publisher
AMER SOC MICROBIOLOGY
Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.71, no.11, pp.6856 - 6862
Abstract
A series of new expression vectors (pPro) have been constructed for the regulated expression of genes in Escherichia coli. The pPro vectors contain the prpBCDE promoter (PprpB) responsible for expression of the propionate catabolic genes (prpBCDE) and prpR encoding the positive regulator of this promoter. The efficiency and regulatory properties of the prpR-PprpB system were measured by placing the gene encoding the green fluorescent protein (gfp) under the control of the inducible PprpB of E. coli. This system provides homogenous expression in individual cells, highly regulatable expression over a wide range of propionate concentrations, and strong expression (maximal 1,500-fold induction) at high propionate concentrations. Since the prpBCDE promoter has CAP-dependent activation, the prpR-PprpB system exhibited negligible basal expression by addition of glucose to the medium.
URI
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DOI
10.1128/AEM.71.11.6856-6862.2005
ISSN
0099-2240
Appears in Collections:
ECHE_Journal Papers
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