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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

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Propionate-regulated high-yield protein production in Escherichia coli

Cited 14 times inthomson ciCited 15 times inthomson ci
Title
Propionate-regulated high-yield protein production in Escherichia coli
Author
Lee, Sung KukKeasling, J.D
Keywords
Expression system; prpBCDE promoter; prpR; Salmonella enterica; T7 promoter
Issue Date
2006-04
Publisher
WILEY-BLACKWELL
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.93, no.5, pp.912 - 918
Abstract
A new expression system containing the Salmonella enterica prpBCDE promoter (PprpB) responsible for expression of the propionate catabolic genes (prp BCDE) and prpR encoding the positive regulator of this promoter has been developed and tested. The main features of the expression system compared to those based on the bacteriophage T7 promoter are low background expression and high induced expression in Escherichia coli strains BL21, BL21(DE3), MG1655, and W3110. In addition, propionate is an inexpensive, simple-to-use, nontoxic inducer that is attractive for large-scale protein production. Hence, this new system is highly complementary to the widely used T7 promoter-driven expression systems.
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DOI
10.1002/bit.20784
ISSN
0006-3592
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ECHE_Journal Papers
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