File Download
There are no files associated with this item.
SFX Link
- Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
- Lee, Sung Kuk
- Synthetic Biology & Metabolic Engineering Lab.
Views & Downloads
Detailed Information
Cited time in 
Cited time in 
Cited time in
Metadata Downloads
Propionate-regulated high-yield protein production in Escherichia coli
- Author(s)
- Lee, Sung Kuk, Keasling, J.D
- Issued Date
- 2006-04
- Citation
- BIOTECHNOLOGY AND BIOENGINEERING, v.93, no.5, pp.912 - 918
- Abstract
- A new expression system containing the Salmonella enterica prpBCDE promoter (PprpB) responsible for expression of the propionate catabolic genes (prp BCDE) and prpR encoding the positive regulator of this promoter has been developed and tested. The main features of the expression system compared to those based on the bacteriophage T7 promoter are low background expression and high induced expression in Escherichia coli strains BL21, BL21(DE3), MG1655, and W3110. In addition, propionate is an inexpensive, simple-to-use, nontoxic inducer that is attractive for large-scale protein production. Hence, this new system is highly complementary to the widely used T7 promoter-driven expression systems.
- Publisher
- WILEY-BLACKWELL
- ISSN
- 0006-3592
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.