Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 5 | - |
dc.citation.startPage | e0285337 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Kim, Sangin | - |
dc.contributor.author | Kim, Yeongjae | - |
dc.contributor.author | Kim, Youyoung | - |
dc.contributor.author | Yoon, Suhyeon | - |
dc.contributor.author | Lee, Kyoo-young | - |
dc.contributor.author | Lee, Yoonsung | - |
dc.contributor.author | Kang, Sukhyun | - |
dc.contributor.author | Myung, Kyungjae | - |
dc.contributor.author | Oh, Chang-Kyu | - |
dc.date.accessioned | 2023-12-21T12:38:08Z | - |
dc.date.available | 2023-12-21T12:38:08Z | - |
dc.date.created | 2023-09-14 | - |
dc.date.issued | 2023-05 | - |
dc.description.abstract | Proliferating cell nuclear antigen (PCNA) is a maestro of DNA replication. PCNA forms a homotrimer and interacts with various proteins, such as DNA polymerases, DNA ligase I (LIG1), and flap endonuclease 1 (FEN1) for faithful DNA replication. Here, we identify the crucial role of Ser46-Leu47 residues of PCNA in maintaining genomic integrity using in vitro, and cell-based assays and structural prediction. The predicted PCNA(& UDelta;SL47) structure shows the potential distortion of the central loop and reduced hydrophobicity. PCNA(& UDelta;SL47) shows a defective interaction with PCNA(WT) leading to defects in homo-trimerization in vitro. PCNA(& UDelta;SL47) is defective in the FEN1 and LIG1 interaction. PCNA ubiquitination and DNA-RNA hybrid processing are defective in PCNA(& UDelta;SL47)-expressing cells. Accordingly, PCNA(& UDelta;SL47)-expressing cells exhibit an increased number of single-stranded DNA gaps and higher levels of & gamma;H2AX, and sensitivity to DNA-damaging agents, highlighting the importance of PCNA Ser46-Leu47 residues in maintaining genomic integrity. | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.18, no.5, pp.e0285337 | - |
dc.identifier.doi | 10.1371/journal.pone.0285337 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.scopusid | 2-s2.0-85159676915 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/66163 | - |
dc.identifier.wosid | 001050599900008 | - |
dc.language | 영어 | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | PCNA Ser46-Leu47 residues are crucial in preserving genomic integrity | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CELL NUCLEAR ANTIGEN | - |
dc.subject.keywordPlus | REPLICATION FACTOR C | - |
dc.subject.keywordPlus | DNA-REPLICATION | - |
dc.subject.keywordPlus | DISTINCT ROLES | - |
dc.subject.keywordPlus | TRANSLESION SYNTHESIS | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | UBIQUITIN | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | POLYMERASES | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.