File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher


Gartner, Anton
DNA Damage Response and Genetic Toxicology
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Full metadata record

DC Field Value Language
dc.citation.number 19 -
dc.citation.startPage gkad776 -
dc.citation.title NUCLEIC ACIDS RESEARCH -
dc.citation.volume 51 - Park, Su Hyung - Kim, Namwoo - Kang, Nalae - Ryu, Eunjin - Lee, Eun A. - Ra, Jae Sun - Gartner, Anton - Kang, Sukhyun - Myung, Kyungjae - Lee, Kyoo-young - 2023-12-21T11:43:55Z - 2023-12-21T11:43:55Z - 2023-10-13 - 2023-10 -
dc.description.abstract Homologous recombination (HR) requires bidirectional end resection initiated by a nick formed close to a DNA double-strand break (DSB), dysregulation favoring error-prone DNA end-joining pathways. Here we investigate the role of the ATAD5, a PCNA unloading protein, in short-range end resection, long-range resection not being affected by ATAD5 deficiency. Rapid PCNA loading onto DNA at DSB sites depends on the RFC PCNA loader complex and MRE11-RAD50-NBS1 nuclease complexes bound to CtIP. Based on our cytological analyses and on an in vitro system for short-range end resection, we propose that PCNA unloading by ATAD5 is required for the completion of short-range resection. Hampering PCNA unloading also leads to failure to remove the KU70/80 complex from the termini of DSBs hindering DNA repair synthesis and the completion of HR. In line with this model, ATAD5-depleted cells are defective for HR, show increased sensitivity to camptothecin, a drug forming protein-DNA adducts, and an augmented dependency on end-joining pathways. Our study highlights the importance of PCNA regulation at DSB for proper end resection and HR. Graphical Abstract -
dc.identifier.bibliographicCitation NUCLEIC ACIDS RESEARCH, v.51, no.19, pp.gkad776 -
dc.identifier.doi 10.1093/nar/gkad776 -
dc.identifier.issn 0305-1048 -
dc.identifier.scopusid 2-s2.0-85175357772 -
dc.identifier.uri -
dc.identifier.wosid 001068403600001 -
dc.language 영어 -
dc.publisher OXFORD UNIV PRESS -
dc.title Short-range end resection requires ATAD5-mediated PCNA unloading for faithful homologous recombination -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.type.docType Article; Early Access -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus PHOSPHORYLATION -
dc.subject.keywordPlus IDENTIFICATION -
dc.subject.keywordPlus DOUBLE-STRAND-BREAK -
dc.subject.keywordPlus REPAIR PATHWAY CHOICE -
dc.subject.keywordPlus DNA-DAMAGE -
dc.subject.keywordPlus HUMAN ELG1 -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus INHIBITORS -
dc.subject.keywordPlus MECHANISM -
dc.subject.keywordPlus CELLS -


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.