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DC Field | Value | Language |
---|---|---|
dc.citation.number | 19 | - |
dc.citation.startPage | gkad776 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 51 | - |
dc.contributor.author | Park, Su Hyung | - |
dc.contributor.author | Kim, Namwoo | - |
dc.contributor.author | Kang, Nalae | - |
dc.contributor.author | Ryu, Eunjin | - |
dc.contributor.author | Lee, Eun A. | - |
dc.contributor.author | Ra, Jae Sun | - |
dc.contributor.author | Gartner, Anton | - |
dc.contributor.author | Kang, Sukhyun | - |
dc.contributor.author | Myung, Kyungjae | - |
dc.contributor.author | Lee, Kyoo-young | - |
dc.date.accessioned | 2023-12-21T11:43:55Z | - |
dc.date.available | 2023-12-21T11:43:55Z | - |
dc.date.created | 2023-10-13 | - |
dc.date.issued | 2023-10 | - |
dc.description.abstract | Homologous recombination (HR) requires bidirectional end resection initiated by a nick formed close to a DNA double-strand break (DSB), dysregulation favoring error-prone DNA end-joining pathways. Here we investigate the role of the ATAD5, a PCNA unloading protein, in short-range end resection, long-range resection not being affected by ATAD5 deficiency. Rapid PCNA loading onto DNA at DSB sites depends on the RFC PCNA loader complex and MRE11-RAD50-NBS1 nuclease complexes bound to CtIP. Based on our cytological analyses and on an in vitro system for short-range end resection, we propose that PCNA unloading by ATAD5 is required for the completion of short-range resection. Hampering PCNA unloading also leads to failure to remove the KU70/80 complex from the termini of DSBs hindering DNA repair synthesis and the completion of HR. In line with this model, ATAD5-depleted cells are defective for HR, show increased sensitivity to camptothecin, a drug forming protein-DNA adducts, and an augmented dependency on end-joining pathways. Our study highlights the importance of PCNA regulation at DSB for proper end resection and HR. Graphical Abstract | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.51, no.19, pp.gkad776 | - |
dc.identifier.doi | 10.1093/nar/gkad776 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.scopusid | 2-s2.0-85175357772 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/65982 | - |
dc.identifier.wosid | 001068403600001 | - |
dc.language | 영어 | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Short-range end resection requires ATAD5-mediated PCNA unloading for faithful homologous recombination | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | DOUBLE-STRAND-BREAK | - |
dc.subject.keywordPlus | REPAIR PATHWAY CHOICE | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | HUMAN ELG1 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CELLS | - |
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