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Kim, Hajin
Single Molecule Biophysics Lab.
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dc.citation.number 9 -
dc.citation.startPage 105081 -
dc.citation.volume 299 - Le, Son Truong - Choi, Seoyun - Lee, Seung-Won - Kim, Hajin - Ahn, Byungchan - 2023-12-21T11:52:25Z - 2023-12-21T11:52:25Z - 2023-07-29 - 2023-09 -
dc.description.abstract RecQ helicases are highly conserved between bacteria and humans. These helicases unwind various DNA structures in the 3′ to 5′. Defective helicase activity elevates genomic instability and is associated with predisposition to cancer and/or premature aging. Recent single-molecule analyses have revealed the repetitive unwinding behavior of RecQ helicases from Escherichia coli to humans. However, the detailed mechanisms underlying this behavior are unclear. Here, we performed single-molecule studies of WRN-1 Caenorhabditis elegans RecQ helicase on various DNA constructs and characterized WRN-1 unwinding dynamics. We showed that WRN-1 persistently repeated cycles of DNA unwinding and rewinding with an unwinding limit of 25 to 31 bp per cycle. Furthermore, by monitoring the ends of the displaced strand during DNA unwinding we demonstrated that WRN-1 reels in the ssDNA overhang in an ATP-dependent manner. While WRN-1 reeling activity was inhibited by a C. elegans homolog of human replication protein A, we found that C. elegans replication protein A actually switched the reiterative unwinding activity of WRN-1 to unidirectional unwinding. These results reveal that reeling-in ssDNA is an intermediate step in the reiterative unwinding process for WRN-1 (i.e., the process proceeds via unwinding-reeling-rewinding). We propose that the reiterative unwinding activity of WRN-1 may prevent extensive unwinding, allow time for partner proteins to assemble on the active region, and permit additional modulation in vivo. -
dc.identifier.bibliographicCitation JOURNAL OF BIOLOGICAL CHEMISTRY, v.299, no.9, pp.105081 -
dc.identifier.doi 10.1016/j.jbc.2023.105081 -
dc.identifier.issn 0021-9258 -
dc.identifier.scopusid 2-s2.0-85169784065 -
dc.identifier.uri -
dc.identifier.wosid 001166279300001 -
dc.language 영어 -
dc.publisher American Society for Biochemistry and Molecular Biology Inc. -
dc.title ssDNA reeling is an intermediate step in the reiterative DNA unwinding activity of the WRN-1 helicase -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.type.docType Article; Early Access -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor C. elegans -
dc.subject.keywordAuthor RecQ helicase -
dc.subject.keywordAuthor reiterative unwinding -
dc.subject.keywordAuthor RPA -
dc.subject.keywordAuthor ssDNA reeling -
dc.subject.keywordAuthor Werner syndrome -
dc.subject.keywordPlus MOLECULE IMAGING REVEALS -
dc.subject.keywordPlus RECQ HELICASE -
dc.subject.keywordPlus STRAND-SEPARATION -
dc.subject.keywordPlus LIFE-SPAN -
dc.subject.keywordPlus REPLICATION -
dc.subject.keywordPlus RECOMBINATION -
dc.subject.keywordPlus HOMOLOG -
dc.subject.keywordPlus REPAIR -
dc.subject.keywordPlus DOMAIN -
dc.subject.keywordPlus WERNER-SYNDROME PROTEIN -


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