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DC Field | Value | Language |
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dc.citation.endPage | 837 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 822 | - |
dc.citation.title | CHEMICAL RESEARCH IN TOXICOLOGY | - |
dc.citation.volume | 36 | - |
dc.contributor.author | Groehler, Arnold S. | - |
dc.contributor.author | Maratova, Asema | - |
dc.contributor.author | Dao, Nhat Mai | - |
dc.contributor.author | Mahkmut, Anuar | - |
dc.contributor.author | Scharer, Orlando D. | - |
dc.date.accessioned | 2023-12-21T12:38:28Z | - |
dc.date.available | 2023-12-21T12:38:28Z | - |
dc.date.created | 2023-07-19 | - |
dc.date.issued | 2023-05 | - |
dc.description.abstract | Cisplatin(CP) is a common antitumor drug that is usedto treatmany solid tumors. The activity of CP is attributed to the formationof DNA-DNA cross-links, which consist of 1,2-intra-, 1,3-intra-,and interstrand cross-links. To better understand how each intrastrandcross-link contributes to the activity of CP, we have developed comprehensiveultraperformance liquid chromatography-selective ion monitoring (UPLC-SIM)assays to quantify 1,2-GG-, 1,2-AG-, 1,3-GCG-, and 1,3-GTG-intrastrandcross-links. The limit of quantitation for the developed assays rangedfrom 5 to 50 fmol or as low as 6 cross-links per 10(8) nucleotides.To demonstrate the utility of the UPLC-SIM assays, we first performed in vitro cross-link formation kinetics experiments. We confirmedthat the 1,2-GG-intrastrand cross-links were the most abundant intrastrandcross-link and formed at a faster rate compared to 1,2-AG- and 1,3-intrastrandcross-links. Furthermore, we investigated the repair kinetics of intrastrandcross-links in CP-treated wild-type and nucleotide excision repair(NER)-deficient U2OS cells. We observed a slow decrease of both 1,2-and 1,3-intrastrand cross-links in wild-type cells and no evidenceof direct repair in the NER-deficient cells. Taken together, we havedemonstrated that our assays are capable of accurately quantifyingintrastrand cross-links in CP-treated samples and can be utilizedto better understand the activity of CP. | - |
dc.identifier.bibliographicCitation | CHEMICAL RESEARCH IN TOXICOLOGY, v.36, no.6, pp.822 - 837 | - |
dc.identifier.doi | 10.1021/acs.chemrestox.2c00308 | - |
dc.identifier.issn | 0893-228X | - |
dc.identifier.scopusid | 2-s2.0-85163184670 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/64836 | - |
dc.identifier.wosid | 001011528000001 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Development of Comprehensive Ultraperformance Liquid Chromatography-High-Resolution Mass Spectrometry Assays to Quantitate Cisplatin-Induced DNA-DNA Cross-Links | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal; Chemistry, Multidisciplinary; Toxicology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy; Chemistry; Toxicology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCISION-REPAIR | - |
dc.subject.keywordPlus | HMG-DOMAIN PROTEIN | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | DAMAGED DNA | - |
dc.subject.keywordPlus | ADDUCTS | - |
dc.subject.keywordPlus | PLATINUM | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | CIS-DIAMMINEDICHLOROPLATINUM(II) | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | GUANINE | - |
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