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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling
  • Calmodulin
  • Plant Defense Mechanism

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Variations in the morphology of rice plants regenerated from protoplasts using different culture procedures - Protoclonal variation of protoplast-derived rice plants

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Title
Variations in the morphology of rice plants regenerated from protoplasts using different culture procedures - Protoclonal variation of protoplast-derived rice plants
Author
Lee, SHShon, YGKim, CYChun, HJCheong, YHKim, ZHChoe, ZRChoi, YJCho, Moo Je
Issue Date
1999
Publisher
SPRINGER
Citation
PLANT CELL TISSUE AND ORGAN CULTURE, v.57, no.3, pp.179 - 187
Abstract
Rice protoplasts were cultured using 4 different culture procedures such as agarose embedding (AE) without feeder cells and the use of filter membranes (MEM), one layer of nylon mesh (MS1), or a double layer of nylon mesh (MS2) with the inclusion of Lolium multiflorum as feeder cells. The protoplast plating efficiency was highest on the MEM, followed by MS2, MS1 and AE. However, plant regeneration frequencies were highest for MS1, followed by MS2, MEM and AE. The protoclonal plants differed in the morphology of leaves, flowers, spikelets, and panicles in comparison to seed-derived plants. They varied in almost every phenotypic characters evaluated. In many cases, the variation was significantly different in characteristics such as plant height, flag leaf length and width and ratio, and in panicle characteristics such as panicle length, number of primary branches, and number of spikelets per panicle. The number of seeds per panicle was greatly reduced in protoclonal plants when compared with seed-derived control plants. The seeds showed also significant differences in grain length and width in comparison to the control plants. Among the 4 groups of protoclonal plants derived from the 4 different culturing procedures themselves, there were also variations in almost all the phenotypic characteristics assessed.
URI
https://scholarworks.unist.ac.kr/handle/201301/6313
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0343882018
DOI
10.1023/A:1006372800761
ISSN
0167-6857
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BIO_Journal Papers
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