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DC Field | Value | Language |
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dc.citation.endPage | 185 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 177 | - |
dc.citation.title | GENE | - |
dc.citation.volume | 178 | - |
dc.contributor.author | Seo, SJ | - |
dc.contributor.author | Kim, Hongtae | - |
dc.contributor.author | Cho, G | - |
dc.contributor.author | Rho, HM | - |
dc.contributor.author | Jung, G | - |
dc.date.accessioned | 2023-12-22T12:38:37Z | - |
dc.date.available | 2023-12-22T12:38:37Z | - |
dc.date.created | 2022-10-17 | - |
dc.date.issued | 1996-10 | - |
dc.description.abstract | The Cu/Zn superoxide dismutase (SOD1) is one of the key enzymes that protect cells against oxidative stress. It catalyzes the dismutation of superoxide radicals (O-2(-)) to oxygen and hydrogen peroxide. To study the transcriptional regulation of the human Cu/Zn SOD gene, we began by analyzing the 1.5-kb upstream region of the gene (see Kim et al., 1994). The element from nucleotides (nt) -116 to -45 increased the transcriptional activity of Cu/Zn SOD. Analyses by DNase I footprinting and electrophoretic mobility shift assay (EMSA) showed that Sp1 binds to the region from nt -104 to -89 and C/EBP-related factors to the region from nt -64 to -55. Studies using two mutant versions of this promoter, in which the Spl and C/EBP-related factor binding sites were deleted, respectively, revealed that Sp1 and C/EBP-related factors activate the transcription of SOD1 gene. An Sp1 expression plasmid, pPacSp1, stimulated the SOD1-linked CAT expression. Cotransfection of the element from nt -116 to -45 with the C/EBP alpha expression vector, pMSV-C/EBP, increased the transcriptional activity of the Cu/Zn SOD in HepG2 cells, but barely in HeLa cells. Because Sp1 is a ubiquitously expressed transcriptional factor, the binding of Sp1 to the proximal upstream region of the Cu/Zn SOD might explain the expression of Cu/Zn SOD in a wide variety of cells. | - |
dc.identifier.bibliographicCitation | GENE, v.178, no.1-2, pp.177 - 185 | - |
dc.identifier.doi | 10.1016/0378-1119(96)00383-6 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.scopusid | 2-s2.0-0030608396 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/59779 | - |
dc.identifier.wosid | A1996VT12600029 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Sp1 and C/EBP-related factor regulate the transcription of human Cu/Zn SOD gene | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | cotransfection | - |
dc.subject.keywordAuthor | DNase I footprinting assay | - |
dc.subject.keywordAuthor | EMSA | - |
dc.subject.keywordAuthor | induction | - |
dc.subject.keywordAuthor | NF-IL6 | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | positive regulatory element | - |
dc.subject.keywordAuthor | transcription factors | - |
dc.subject.keywordPlus | ENHANCER BINDING-PROTEIN | - |
dc.subject.keywordPlus | MANGANESE SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | LIVER NUCLEAR-PROTEIN | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | ELEMENT | - |
dc.subject.keywordPlus | FAMILY | - |
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