There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 19791 | - |
dc.citation.number | 31 | - |
dc.citation.startPage | 19786 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 273 | - |
dc.contributor.author | Lee, H | - |
dc.contributor.author | Kim, Hongtae | - |
dc.contributor.author | Yun, Y | - |
dc.date.accessioned | 2023-12-22T12:35:57Z | - |
dc.date.available | 2023-12-22T12:35:57Z | - |
dc.date.created | 2022-10-17 | - |
dc.date.issued | 1998-07 | - |
dc.description.abstract | Here, we established the inhibitory mechanism of p53 on hepatitis B viral gene expression using HepG2 cells. Our results are as follows, First, p53 down-regulated the activities of all four promoters of hepatitis B virus (HBV), suggestive of the presence of a common element mediating the p53-dependent transcriptional repression. Second, employing the 5'-deletion constructs of the pregenomic/core promoter, the liver-specific enhancer II region was localized as a target for the p53-mediated transcriptional repression. Third, in a detailed analysis of the enhancer II region, the 5'-proximal 31-base pair region was defined as a p53-repressible element. Throughout the study, p53-mediated repression was rescued upon coexpression of the X-gene product, HBx. Finally, in an electrophoretic mobility shift assay, the defined p53-repressible element did not bind purified p53 directly, but shifted three bands in HepG2 nuclear extract, two of which was supershifted upon addition of p53 monoclonal antibody. These results display a novel mechanism of p53 dependent transcriptional repression in which p53 negatively regulates the viral-specific DNA enhancer through protein to protein interaction with an enhancer-binding protein. At the same time, the results indicate that p53 plays a defensive role against HBV by transcriptionally repressing the HBV core promoter through liver-specific enhancer II and HBx is required to counteract this inhibitory function of p53. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.31, pp.19786 - 19791 | - |
dc.identifier.doi | 10.1074/jbc.273.31.19786 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-15144352326 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/59777 | - |
dc.identifier.wosid | 000075125200062 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Liver-specific enhancer II is the target for the p53-mediated inhibition of hepatitis B viral gene expression | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | VIRUS HBX PROTEIN | - |
dc.subject.keywordPlus | RNA POLYMERASE-II | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | TRANSCRIPTION INITIATION | - |
dc.subject.keywordPlus | DNA-BINDING | - |
dc.subject.keywordPlus | WILD-TYPE | - |
dc.subject.keywordPlus | X-GENE | - |
dc.subject.keywordPlus | CORE PROMOTER | - |
dc.subject.keywordPlus | 2ND ENHANCER | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.