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Park, Jung-Hoon
Bio-Optics Lab
Research Interests
  • Biomedical optics, wavefront shaping, In-vivo deep tissue imaging


High-throughput high-dynamic range imaging by spatiotemporally structured illumination

DC Field Value Language Woo, Taeseong ko Kim, Hye Yun ko Kim, Su Yeon ko Hwang, Byungjae ko Ahn, Cheolwoo ko Kwon, Seok-Kyu ko Kim, Jae-Ick ko Park, Jung-Hoon ko 2022-10-14T03:44:31Z - 2022-10-11 ko 2022-10 ko
dc.identifier.citation APL PHOTONICS, v.7, no.10, pp.106106 ko
dc.identifier.issn 2378-0967 ko
dc.identifier.uri -
dc.description.abstract Recent advances in biochemistry and optics have enabled observation of the faintest signals from even single molecules. However, although biological samples can have varying degrees of fluorescence expression ranging from a single to thousands of fluorescent molecules in an observation volume, the detection range is fundamentally limited by the dynamic range (DR) of current detectors. In other words, for many biological systems where faint and strong signal sources coexist, traditional imaging methods make a compromise and end up choosing a limited target signal range to be quantitatively measured while other signal levels are either lost beneath the background noise or saturated. The DR can be extended by taking multiple images with varying exposures, which however, severely restricts data throughput. To overcome this limitation, we introduce structured illumination high dynamic range (SI-HDR) imaging which enables real time HDR imaging with a single measurement. We demonstrate the wide and easy applicability of the method by realizing various applications such as high throughput gigapixel imaging of mouse brain slice, quantitative analysis of neuronal mitochondria structures, and fast 3D volumetric HDR imaging. ko
dc.language 영어 ko
dc.publisher AIP Publishing LLC | American Institute of Physics ko
dc.title High-throughput high-dynamic range imaging by spatiotemporally structured illumination ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-85141881815 ko
dc.identifier.wosid 000878166400001 ko
dc.type.rims ART ko
dc.identifier.doi 10.1063/5.0099780 ko
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