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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

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Title
Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis
Author
Kim, Chi-JuKuczler, Morgan D.Dong, LiangKim, JunyoungAmend, Sarah R.Cho, Yoon-KyoungPienta, Kenneth J.
Issue Date
2022-02
Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
Citation
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v.2022, no.180, pp.e62836
Abstract
There is a need for practical assays to visualize and quantify the cells' extracellular vesicle (EV) uptake. EV uptake plays a role in intercellular communication in various research fields; cancer biology, neuroscience, and drug delivery. Many EV uptake assays have been reported in the literature; however, there is a lack of practical, detailed experimental methodology. EV uptake can be assessed by fluorescently labeling EVs to detect their location within cells. Distinguishing between internalized EVs in cells and the superficial EVs on cells is difficult, yet critical, to accurately determine the EV uptake. Therefore, an assay that efficiently quantifies EV uptake through three-dimensional (3D) fluorescence confocal microscopy is proposed in this work. Fluorescently labeled EVs were prepared using a nano-filtration-based microfluidic device, visualized by 3D confocal microscopy, and then analyzed through advanced image-processing software. The protocol provides a robust methodology for analyzing EVs on a cellular level and a practical approach for efficient analysis.
URI
https://scholarworks.unist.ac.kr/handle/201301/58410
URL
https://www.jove.com/kr/t/62836/extracellular-vesicle-uptake-assay-via-confocal-microscope-imaging
DOI
10.3791/62836
ISSN
1940-087X
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