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Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal Ions <i>In Vivo</i> Using High-Intensity Focused Ultrasound

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Title
Noninvasive and Spatiotemporal Control of DNAzyme-Based Imaging of Metal Ions In Vivo</i> Using High-Intensity Focused Ultrasound
Author
Wang, XiaojingKim, GunChu, James L.Song, TingjieYang, ZhenglinGuo, WeijieShao, XiangliOelze, Michael L.Li, King C.Lu, Yi
Issue Date
2022-03
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.144, no.13, pp.5812 - 5819
Abstract
Detecting metal ions invivo with a high spatiotemporal resolution is critical to understanding the roles of the metal ions in both healthy and disease states. Although spatiotemporal controls of metal-ion sensors using light have been demonstrated, the lack of penetration depth in tissue and in vivo has limited their application. To overcome this limitation, we herein report the use of high-intensity focused ultrasound (HIFU) to remotely deliver on-demand, spatiotemporally resolved thermal energy to activate the DNAzyme sensors at the targeted region both in vitro and in vivo. A Zn2+-selective DNAzyme probe is inactivated by a protector strand to block the formation of catalytic enzyme structure, which can then be activated by an HIFU-induced increase in the local temperature. With this design, Zn2+-specific fluorescent resonance energy transfer (FRET) imaging has been demonstrated by the new DNAzyme-HIFU probes in both HeLa cells and mice. The current method can be applied to monitor many other metal ions for in vivo imaging and medical diagnosis using metal-specific DNAzymes that have either been obtained or can be selected using in vitro selection.
URI
https://scholarworks.unist.ac.kr/handle/201301/58299
URL
https://pubs.acs.org/doi/10.1021/jacs.1c11543
DOI
10.1021/jacs.1c11543
ISSN
0002-7863
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