World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays
Cited 35 times inCited 41 times in
- World-to-chip microfluidic interface with built-in valves for multichamber chip-based PCR assays
- Oh, KW; Park, CS; Namkoong, K; Kim, J; Ock, KS; Kim, S; Kim, YA; Cho, Yoon-Kyoung; Ko, C
- Issue Date
- ROYAL SOC CHEMISTRY
- LAB ON A CHIP, v.5, no.8, pp.845 - 850
- We report a practical world-to-chip microfluidic interfacing method with built-in valves suitable for microscale multichamber chip-based assays. One of the primary challenges associated with the successful commercialization of fully integrated microfluidic systems has been the lack of reliable world-to-chip microfluidic interconnections. After sample loading and sealing, leakage tests were conducted at 100 °C for 30 min and no detectable leakage flows were found during the test for 100 microchambers. To demonstrate the utility of our world-to-chip microfluidic interface, we designed a microscale PCR chip with four chambers and performed PCR assays. The PCR results yielded a 100% success rate with no contamination or leakage failures. In conclusion, we have introduced a simple and inexpensive microfluidic interfacing system for both sample loading and sealing with no dead volume, no leakage flow and biochemical compatibility.
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.