Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 2311 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 12 | - |
dc.contributor.author | Lee, Daesoon | - |
dc.contributor.author | Bhardwaj, Jyoti | - |
dc.contributor.author | Jang, Jaesung | - |
dc.date.accessioned | 2023-12-21T14:39:03Z | - |
dc.date.available | 2023-12-21T14:39:03Z | - |
dc.date.created | 2022-02-02 | - |
dc.date.issued | 2022-02 | - |
dc.description.abstract | Many studies have been conducted on measuring avian influenza viruses and their hemagglutinin (HA) antigens via electrochemical principles; most of these studies have used gold electrodes on ceramic, glass, or silicon substrates, and/or labeling for signal enhancement. Herein, we present a paper-based immunosensor for label-free measurement of multiple avian influenza virus (H5N1, H7N9, and H9N2) antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes. These flexible electrodes on a paper substrate can complement the physical weakness of the paper-based sensors when wetted, without affecting flexibility. The relative standard deviation of the peak currents was 1.88% when the electrodes were repeatedly bent and unfolded twenty times with deionized water provided each cycle, showing the stability of the electrodes. For the detection of HA antigens, approximately 10-μl samples (concentration: 100 pg/ml–100 ng/ml) were needed to form the antigen–antibody complexes during 20–30 min incubation, and the immune responses were measured via differential pulse voltammetry. The limits of detections were 55.7 pg/ml (0.95 pM) for H5N1 HA, 99.6 pg/ml (1.69 pM) for H7N9 HA, and 54.0 pg/ml (0.72 pM) for H9N2 HA antigens in phosphate buffered saline, and the sensors showed good selectivity and reproducibility. Such paper-based sensors are economical, flexible, robust, and easy-to-manufacture, with the ability to detect several avian influenza viruses. | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.12, pp.2311 | - |
dc.identifier.doi | 10.1038/s41598-022-06101-1 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.scopusid | 2-s2.0-85124500377 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/57145 | - |
dc.identifier.url | https://www.nature.com/articles/s41598-022-06101-1 | - |
dc.identifier.wosid | 000754021000074 | - |
dc.language | 영어 | - |
dc.publisher | NATURE RESEARCH | - |
dc.title | Paper-based electrochemical immunosensor for label-free detection of multiple avian influenza virus antigens using flexible screen-printed carbon nanotube-polydimethylsiloxane electrodes | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | IMPEDANCE IMMUNOSENSOR | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | LOW-COST | - |
dc.subject.keywordPlus | H5N1 | - |
dc.subject.keywordPlus | HEMAGGLUTIN | - |
dc.subject.keywordPlus | INIMMUNOASSAY | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | NANOBEADS | - |
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