BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.206, no.1, pp.194 - 200
Abstract
Phospholipase C (PLC) has been known to be a key effector protein in signal transduction pathway for cell proliferation and differentiation. Here, we report the transcriptional regulation of PLC-γ1 during myogenic differentiation. Both PLC-γ1 mRNA and protein levels were increased during myogenesis. The activity of PLC-γ1 was also comparable to the amount estimated by Western blot. Comparing promoter activities of various PLC-γ1 gene upstream regions in myoblast and myotube, the responsible region was mapped between -551 and -480. From these results, we conclude that the transcription of PLC-γ1 is increased by the control of GPE1 region of PLC-γ1 gene during myogenesis.