File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

서판길

Suh, Pann-Ghill
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Trp-Lys-Tyr-Met-Val-D-Met stimulates superoxide generation and killing of Staphylococcus aureus via phospholipase D activation in human monocytes

Author(s)
Bae, YSJu, SAKim, JYSeo, Jeong KonBaek, SHKwak, JYKim, BSSuh, Pann-GhillRyu, SH
Issued Date
1999-02
URI
https://scholarworks.unist.ac.kr/handle/201301/5703
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033047887
Citation
JOURNAL OF LEUKOCYTE BIOLOGY, v.65, no.2, pp.241 - 248
Abstract
Among the phagocytic leukocytes, monocytes have the important role of clearing out parasitic microorganisms. They accomplish this through production of toxic metabolites of oxygen. Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), a peptide that stimulates phosphoinositide (PI) hydrolysis in human leukocytes, including monocytes, binds to a unique cell surface receptor and stimulates superoxide generation, killing of Staphylococcus aureus, and activation of phospholipase D (PLD) in human monocytes. Preincubation of the cells with a PI-specific phospholipase C (PLC) inhibitor (U-73122), protein kinase C inhibitor (GF109203X), or intracellular Ca 2+ chelator (BAPTA/AM) before the peptide stimulus totally inhibits the peptide-induced PLD activation and superoxide generation. On the other hand, tyrosine kinase inhibitor genistein only partially inhibits the peptide-induced processes. The peptide-induced bacteria killing activity shares regulatory mechanisms for PLD activation with the superoxide generation, which is inhibited in the presence of 1-butanol. We suggest that the peptide stimulates PLD downstream of PLC activation and PLD activation in turn is essential for the peptide- induced immunological functions such as the superoxide generation and killing of bacteria by human monocytes.
Publisher
FEDERATION AMER SOC EXP BIOL
ISSN
0741-5400

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.