There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 3 | - |
dc.citation.startPage | 2101239 | - |
dc.citation.title | SMALL METHODS | - |
dc.citation.volume | 6 | - |
dc.contributor.author | Lee, Min Seok | - |
dc.contributor.author | Hyun, Hwi | - |
dc.contributor.author | Park, Inwon | - |
dc.contributor.author | Kim, Sungho | - |
dc.contributor.author | Jang, Dong-Hyun | - |
dc.contributor.author | Kim, Seonghye | - |
dc.contributor.author | Im, Jae-Kyeong | - |
dc.contributor.author | Kim, Hajin | - |
dc.contributor.author | Lee, Jae Hyuk | - |
dc.contributor.author | Kwon, Taejoon | - |
dc.contributor.author | Kang, Joo H. | - |
dc.date.accessioned | 2023-12-21T14:42:23Z | - |
dc.date.available | 2023-12-21T14:42:23Z | - |
dc.date.created | 2022-01-15 | - |
dc.date.issued | 2022-01 | - |
dc.description.abstract | The current diagnosis of bacteremia mainly uses blood culture, which is insufficient to offer rapid and quantitative determination of pathogens in blood. Here, we report a quantitative and sequential multiplexed fluorescence in situ hybridization in a microfluidic device (µFISH) that enables early and rapid (2-hour) diagnosis of bacteremia without prior blood culture. Mannose-binding lectin-coated magnetic nanoparticles enrich a broad range of pathogens, and µFISH enables identification and quantification of the magnetically confined bacteria. We detect Escherichia coli (E. coli) and measure their relative proportions to universal bacteria levels in the bacteremic blood of a porcine model and human whole blood collected from E. coli-infected patients, which was elusive with the conventional bacteremia diagnosis methods. Thus, µFISH can be used as a versatile tool to rapidly identify pathogens and further assess the number of both culturable and non-culturable bacteria in biological and environmental samples. | - |
dc.identifier.bibliographicCitation | SMALL METHODS, v.6, no.3, pp.2101239 | - |
dc.identifier.doi | 10.1002/smtd.202101239 | - |
dc.identifier.issn | 2366-9608 | - |
dc.identifier.scopusid | 2-s2.0-85124071217 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/56880 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/smtd.202101239 | - |
dc.identifier.wosid | 000750169900001 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables early detection of human bacteremia | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical;Nanoscience & Nanotechnology;Materials Science, Multidisciplinary | - |
dc.relation.journalResearchArea | Chemistry;Science & Technology - Other Topics;Materials Science | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.subject.keywordAuthor | bacteremia diagnosis | - |
dc.subject.keywordAuthor | fluorescence in situ hybridization (FISH) | - |
dc.subject.keywordAuthor | magnetic nanoparticles | - |
dc.subject.keywordAuthor | microfluidic FISH | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | BLOOD-CULTURE | - |
dc.subject.keywordPlus | TELOMERE LENGTH | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | TIME | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | POSITIVITY | - |
dc.subject.keywordPlus | SEPSIS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | SEQUENCES | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.