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Myung, Kyungjae
Center for Genomic Integrity
Research Interests
  • DNA Replication, DNA Repair, DNA Recombination, DNA Damage Response, cancer, aging

Characterization of subcellular localization of eukaryotic clamp loader/unloader and its regulatory mechanism

DC Field Value Language Park, Su Hyung ko Kim, Seong-jung ko Myung, Kyungjae ko Lee, Kyoo-young ko 2021-12-24T00:43:37Z - 2021-12-09 ko 2021-11 ko
dc.identifier.citation SCIENTIFIC REPORTS, v.11, no.1, pp.21817 ko
dc.identifier.issn 2045-2322 ko
dc.identifier.uri -
dc.description.abstract Proliferating cell nuclear antigen (PCNA) plays a critical role as a processivity clamp for eukaryotic DNA polymerases and a binding platform for many DNA replication and repair proteins. The enzymatic activities of PCNA loading and unloading have been studied extensively in vitro. However, the subcellular locations of PCNA loaders, replication complex C (RFC) and CTF18-RFC-like-complex (RLC), and PCNA unloader ATAD5-RLC remain elusive, and the role of their subunits RFC2-5 is unknown. Here we used protein fractionation to determine the subcellular localization of RFC and RLCs and affinity purification to find molecular requirements for the newly defined location. All RFC/RLC proteins were detected in the nuclease-resistant pellet fraction. RFC1 and ATAD5 were not detected in the non-ionic detergent-soluble and nuclease-susceptible chromatin fractions, independent of cell cycle or exogenous DNA damage. We found that small RFC proteins contribute to maintaining protein levels of the RFC/RLCs. RFC1, ATAD5, and RFC4 co-immunoprecipitated with lamina-associated polypeptide 2 (LAP2) alpha which regulates intranuclear lamin A/C. LAP2 alpha knockout consistently reduced detection of RFC/RLCs in the pellet fraction, while marginally affecting total protein levels. Our findings strongly suggest that PCNA-mediated DNA transaction occurs through regulatory machinery associated with nuclear structures, such as the nuclear matrix. ko
dc.language 영어 ko
dc.publisher NATURE PORTFOLIO ko
dc.title Characterization of subcellular localization of eukaryotic clamp loader/unloader and its regulatory mechanism ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-85118656870 ko
dc.identifier.wosid 000716101700034 ko
dc.type.rims ART ko
dc.identifier.doi 10.1038/s41598-021-01336-w ko
dc.identifier.url ko
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