Full metadata record
DC Field | Value | Language |
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dc.citation.number | 1 | - |
dc.citation.startPage | 1681 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 12 | - |
dc.contributor.author | Lee, Yang-Ha | - |
dc.contributor.author | Yu, Eunsoo | - |
dc.contributor.author | Park, Cheol-Min | - |
dc.date.accessioned | 2023-12-21T16:09:48Z | - |
dc.date.available | 2023-12-21T16:09:48Z | - |
dc.date.created | 2021-04-15 | - |
dc.date.issued | 2021-03 | - |
dc.description.abstract | Site-selective modification of oligonucleotides serves as an indispensable tool in many fields of research including research of fundamental biological processes, biotechnology, and nanotechnology. Here we report chemo- and regioselective modification of oligonucleotides based on rhodium(I)-carbene catalysis in a programmable fashion. Extensive screening identifies a rhodium(I)-catalyst that displays robust chemoselectivity toward base-unpaired guanosines in single and double-strand oligonucleotides with structurally complex secondary structures. Moreover, high regioselectivity among multiple guanosines in a substrate is achieved by introducing guanosine-bulge loops in a duplex. This approach allows the introduction of multiple unique functional handles in an iterative fashion, the utility of which is exemplified in DNA-protein cross-linking in cell lysates. The methods for investigation of DNA-binding proteins require site-selective chemical modifications to be introduced into oligonucleotides. Here, the authors report a chemo- and regioselective method for the modification of unpaired guanosines in single- and double-stranded oligonucleotides, based on Rh(I)-carbene catalysis. | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.12, no.1, pp.1681 | - |
dc.identifier.doi | 10.1038/s41467-021-21839-4 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.scopusid | 2-s2.0-85102564780 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/52748 | - |
dc.identifier.url | https://www.nature.com/articles/s41467-021-21839-4 | - |
dc.identifier.wosid | 000629990000001 | - |
dc.language | 영어 | - |
dc.publisher | NATURE RESEARCH | - |
dc.title | Programmable site-selective labeling of oligonucleotides based on carbene catalysis | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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