One step pathogen specific DNA extraction from whole blood

Abstract

We could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood utilizing centrifugal microfluidics on a polymer based CD platform. By combining the TS-LIMBS (Target Separation and Laser-Irradiated Magnetic Bead System) and centrifugal microfluidics using the novel LIFM (The Laser Irradiated Ferrowax Microvalves), DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) were conducted. The total process was finished within 12 minutes with only one manual step of loading 100 μL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD was as good as that of the samples prepared in conventional bench top method.