dc.citation.conferencePlace |
US |
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dc.citation.title |
American Society for Cell Biology 48th Anuual Meeting |
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dc.contributor.author |
Park, Chan Young |
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dc.contributor.author |
Hoover, P.J. |
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dc.contributor.author |
Mullins, F.M. |
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dc.contributor.author |
Bachhawat, P. |
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dc.contributor.author |
Covington, E.D. |
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dc.contributor.author |
Raunser, S. |
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dc.contributor.author |
Walz, T. |
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dc.contributor.author |
Garcia, K.C. |
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dc.contributor.author |
Dolmetsch, R.E. |
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dc.contributor.author |
Lewis, R.S. |
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dc.date.accessioned |
2023-12-20T04:11:01Z |
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dc.date.available |
2023-12-20T04:11:01Z |
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dc.date.created |
2014-12-23 |
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dc.date.issued |
2008-12-17 |
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dc.description.abstract |
Store-operated Ca2+ channels activated by the depletion of Ca2+ from the endoplasmic reticulum (ER) are a major Ca2+ entry pathway in nonexcitable cells and are essential for T cell activation and adaptive immunity. After store depletion, the ER Ca2+ sensor STIM1 and the CRAC channel protein Orai1 redistribute to ER-plasma membrane (PM) junctions, but the fundamental issue of how STIM1 activates the CRAC channel at these sites is unresolved. Here, we identify a minimal, highly conserved 107-aa CRAC activation domain (CAD) of STIM1 that binds directly to the N and C termini of Orai1 to open the CRAC channel. Purified CAD forms a tetramer that clusters CRAC channels, but analysis of STIM1 mutants reveals that channel clustering is not sufficient for channel activation. These studies establish a molecular mechanism for store-operated Ca2+ entry in which the direct binding of STIM1 to Orai1 drives the accumulation and the activation of CRAC channels at ER-PM junctions. |
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dc.identifier.bibliographicCitation |
American Society for Cell Biology 48th Anuual Meeting |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/51709 |
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dc.publisher |
American Society for Cell Biology 48th Anuual Meeting |
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dc.title |
STIM1 Clusters and Activates CRAC Channels via Direct Binding of a Cytosolic Domain to Orai1 |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2008-12-17 |
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