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Quantitative analysis of blood cells from microscopic images using convolutional neural network

Tessema, Abel WorkuMohammed, Mohammed AliySimegn, Gizeaddis LamesginKwa, Timothy Chung
Issued Date
Medical and Biological Engineering and Computing, v.59, no.1, pp.143 - 152
Blood cell count provides relevant clinical information about different kinds of disorders. Any deviation in the number of blood cells implies the presence of infection, inflammation, edema, bleeding, and other blood-related issues. Current microscopic methods used for blood cell counting are very tedious and are highly prone to different sources of errors. Besides, these techniques do not provide full information related to blood cells like shape and size, which play important roles in the clinical investigation of serious blood-related diseases. In this paper, deep learning-based automatic classification and quantitative analysis of blood cells are proposed using the YOLOv2 model. The model was trained on 1560 images and 2703-labeled blood cells with different hyper-parameters. It was tested on 26 images containing 1454 red blood cells, 159 platelets, 3 basophils, 12 eosinophils, 24 lymphocytes, 13 monocytes, and 28 neutrophils. The network achieved detection and segmentation of blood cells with an average accuracy of 80.6% and a precision of 88.4%. Quantitative analysis of cells was done following classification, and mean accuracy of 92.96%, 91.96%, 88.736%, and 92.7% has been achieved in the measurement of area, aspect ratio, diameter, and counting of cells respectively.
Institute of Electrical Engineers
Keyword (Author)
Blood cellsDetectionMorphological parametersYOLOv2


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